Human CCL7/MCP‑3/MARC Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF282
Key Product Details
Species Reactivity
Human
Applications
ELISA Detection (Matched Antibody Pair), Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL7/MCP-3 (R&D Systems, Catalog # 282-P3)
Gln34-Leu109
Accession # Q7Z7Q8
Gln34-Leu109
Accession # Q7Z7Q8
Specificity
Detects human CCL7/MCP-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Eotaxin, recombinant mouse (rm) JE, rhI-309, rhMCP-1, rhMCP-2, rmMCP-3, rhMCP-4, and rmMCP-5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human CCL7/MCP‑3/MARC Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3)
Sample: Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3)
Human CCL7/MCP-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL7/MCP-3/MARC
MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.
CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.
Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Alternate Names
MARC, MCP-3
Gene Symbol
CCL7
UniProt
Additional CCL7/MCP-3/MARC Products
Product Documents for Human CCL7/MCP‑3/MARC Biotinylated Antibody
Product Specific Notices for Human CCL7/MCP‑3/MARC Biotinylated Antibody
For research use only
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