Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either Mouse Anti-Human CCR6 Monoclonal Antibody (Catalog # MAB195R, filled histogram) or Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Goat anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

CCR6 in Human Spleen.

CCR6 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CCR6 Monoclonal Antibody (Catalog # MAB195R) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CCR6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: CD19+ Human PBMCs

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Alternate Names

CC-CKR-6, CCR6, CD196, CKR-L3, CKRL3, CMKBR6, DRY6, GPR-CY4, GPR29, GPRCY4, STRL22

Entrez Gene IDs

1235 (Human); 12458 (Mouse); 308163 (Rat)

Gene Symbol

CCR6

UniProt

P51684

Additional CCR6 Products

Product Documents for Human CCR6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCR6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CCR6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB195R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CCR6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CCR6 Antibody

Detection of CCR6 in CD19+ Human PBMCs by Flow Cytometry.