Human peripheral blood mononuclear cells (PBMCs) were stained with PE-conjugated Mouse anti-Human CD4 Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse anti-Human CCR7 Biotinylated Monoclonal Antibody (Catalog # FAB197B) or (B) Mouse IgG2A isotype control antibody (Catalog # IC003B) followed by APC-conjugated Streptavidin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CCR7 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: Human PBMCs

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Alternate Names

BLR2, CC-CKR-7, CCR7, CD197, CDw197, CMKBR7, EBI1

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Gene Symbol

CCR7

UniProt

AAA58615

Additional CCR7 Products

Product Documents for Human CCR7 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCR7 Biotinylated Antibody

For research use only

Citations
Reviews

Human CCR7 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB197B

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CCR7 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CCR7 Biotinylated Antibody

Detection of CCR7 in Human PBMCs by Flow Cytomtetry.