Human CCR7 Fluorescein-conjugated Antibody

Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR7 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB197F) or (B) Mouse IgG_2AFluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry.

The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of Human CCR7 by Flow Cytometry

Frequency of peripheral T lymphocytes in hip fracture patients with and without depressive symptoms versus healthy controls. (a) The percentage of CD3+ve T cells in healthy controls: n = 37; hip fracture patients without depression (HF): n = 38 and hip fracture patients with depressive symptoms (HF + D): n = 29.The solid bar represents the mean value. (b) The mean absolute number of T cells in healthy controls: n = 20; hip fracture patients without depression (HF): n = 19 and hip fracture patients with depressive symptoms (HF + D): n = 15. Data are mean ± SEM. *p < .05 and **p < .01. Representative flow cytometry plots and gating strategy of PBMCs from a young individual stained with anti-CD3 and (c) anti-CD4, and CD8, (d) and anti-CD45RA and -CCR7 (e and f). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25628751), licensed under a CC-BY license. Not internally tested by R&D Systems.