MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R, filled histogram) or Mouse IgG_2AFlow Cytometry Isotype Control (Catalog # MAB003) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR9 in HEK293 Human Cell Line Transfected with Human CCR9 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human CCR9 and eGFP was stained with (A) Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R) or (B) Mouse IgG_2Aisotype control antibody (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

CCR9 in MOLT‑4 Human Cell Line.

CCR9 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CCR9 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: MOLT-4 human acute lymphoblastic leukemia cell line and HEK293 Human Cell Line Transfected with Human CCR9 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Alternate Names

CCR9, CD199, CDw199

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Gene Symbol

CCR9

UniProt

P51686

Additional CCR9 Products

Product Documents for Human CCR9 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCR9 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CCR9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1791R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CCR9 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CCR9 Antibody

Detection of CCR9 in Human MOLT-4 cells by Flow Cytometry.