Human CD11c Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB17771
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 538207
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD11c
aa 20-1107
Accession # P20702
aa 20-1107
Accession # P20702
Specificity
Detects human CD11c in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Integrin alpha1, 2, 4, 5, 6, 8, 9, 10, 11, L, M, rhIntegrin alpha2b/ beta3, alpha3/ beta1, alpha6X1/ beta4, alpha7X2/ beta1, alphaD/ beta2, alphaE/ beta7, alphaV/ beta6, recombinant mouse Integrin alphaX, and alpha7X2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD11c Antibody
Detection of Human CD11c by Western Blot.
Western blot shows lysates of human mature myeloid dendritic cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CD11c Monoclonal Antibody (Catalog # MAB17771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD11c at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1with 0.05% Tween® 20 in the blotting buffer.
Detection of CD11c in human tonsil.
CD11c was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD11c Monoclonal Antibody (Catalog # MAB17771) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CD11c Antibody
Application
Recommended Usage
Immunohistochemistry
0.5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Western Blot
2 µg/mL
Sample: Human mature myeloid dendritic cells
Sample: Human mature myeloid dendritic cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11c
CD11c, also known as Integrin alphaX, is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta2, also known as CD18. The CD11c/CD18 complex, also called CR4 (complement receptor type 4) is expressed on macrophages, dendritic cells and hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells. It binds fibrinogen and has been reported to be a receptor for complement component iC3b (1‑3). The human CD11c extracellular domain (amino acids 20-1107) shares 70 - 76% amino acid sequence identity with mouse, rat and canine CD11c.
References
- Hogg, N. et al. (1986) Eur. J. Immunol. 16(3):240.
- Knapp, W.B. et al. eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
- Stacker, S.A. and T.A. Springer, J. Immunol. (1991) 146(2):648.
Alternate Names
CD11c, Integrin alpha X, ITGAX, p150,95 alpha
Gene Symbol
ITGAX
UniProt
Additional CD11c Products
Product Documents for Human CD11c Antibody
Product Specific Notices for Human CD11c Antibody
For research use only
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