Human CD160 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6700A

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 688327

View all CD160 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD160
Ile27-Ser159
Accession # O95971

Specificity

Detects human CD160 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD160 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD160 APC-conjugated Antibody

Detection of CD160 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD160 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD160 APC-conjugated Monoclonal Antibody (Catalog # FAB6700A) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.
Detection of Human CD160 by Flow Cytometry

Detection of Human CD160 by Flow Cytometry

Upregulation of immune checkpoint ligands on T cells after dendritic cell stimulation. T cells of 7–14 healthy donor were cocultured with autologous TLR-3-DCs pulsed with CMV, EBV, influenza, tetanus (CEFT) peptide pool or with CEFT peptide pool alone. Expression of various inhibitory checkpoint molecules was analyzed by flow cytometry. The percentage of positive cells is presented as box-and-whisker plots for CD4+(A) and for CD8+(B) T cells. *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fimmu.2018.00385/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CD160 by Flow Cytometry

Detection of Human CD160 by Flow Cytometry

Upregulation of immune checkpoint ligands on T cells after dendritic cell stimulation. T cells of 7–14 healthy donor were cocultured with autologous TLR-3-DCs pulsed with CMV, EBV, influenza, tetanus (CEFT) peptide pool or with CEFT peptide pool alone. Expression of various inhibitory checkpoint molecules was analyzed by flow cytometry. The percentage of positive cells is presented as box-and-whisker plots for CD4+(A) and for CD8+(B) T cells. *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fimmu.2018.00385/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD160 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD160

CD160 (also BY55) is a 27 kDa member of the immunoglobulin superfamily of molecules.  It is expressed on select hematopoietic cell types, including CD56dim CD16+ cytotoxic NK cells, CD8+ CD28- effector T cells, delta/ gamma T cells, and restricted CD4+ T cells.  It is a receptor for HLA-C molecules, and its engagement induces CD160+ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program.  Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27‑181).  It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one V‑type Ig-like domain (aa 27-122).  CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180-Leu181, and shows a 20 aa TM segment between aa 163-182.  The TM form appears to have a splice variant that lacks aa 25-133.  Over aa 27-159, human CD160 shares 62% aa identity with mouse CD160.  

Alternate Names

BY55, CD160, NK1, NK28

Entrez Gene IDs

11126 (Human); 54215 (Mouse); 502585 (Rat); 102117412 (Cynomolgus Monkey)

Gene Symbol

CD160

UniProt

O95971

Additional CD160 Products

Product Documents for Human CD160 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD160 APC-conjugated Antibody

For research use only

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