Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD160 PE-conjugated Monoclonal Antibody (Catalog # FAB6700P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). View our protocol for Staining Membrane-associated Proteins.

Detection of Human Human CD160 PE-conjugated Antibody by Flow Cytometry

Phenotype of CD8 and CD4 T cells stimulated with either non-ubiquitinated or ubiquitinated constructs.MoDCs were transduced with either the non-ubiquitinated (0x, hatched bar) or ubiquitinated (1x, grey bar) HIV-1 CN54 gag constructs and co-cultured with autologous naïve T cells. After each subsequent week in culture, cells were removed and stained with antibodies to A) CD8 or B) CD4 and the indicated surface markers. Cells were analysed by flow cytometry, and the box plots represent the median with upper and lower quartiles, and whiskers represent the min and max values of 9 donors. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24505475), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD160 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD160

CD160 (also BY55) is a 27 kDa member of the immunoglobulin superfamily of molecules. It is expressed on select hematopoietic cell types, including CD56^dim CD16⁺ cytotoxic NK cells, CD8⁺ CD28^- effector T cells, delta/ gamma T cells, and restricted CD4⁺ T cells. It is a receptor for HLA-C molecules, and its engagement induces CD160⁺ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program. Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27‑181). It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one V‑type Ig-like domain (aa 27-122). CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180-Leu181, and shows a 20 aa TM segment between aa 163-182. The TM form appears to have a splice variant that lacks aa 25-133. Over aa 27-159, human CD160 shares 62% aa identity with mouse CD160.

Alternate Names

BY55, CD160, NK1, NK28

Entrez Gene IDs

11126 (Human); 54215 (Mouse); 502585 (Rat); 102117412 (Cynomolgus Monkey)

Gene Symbol

CD160

UniProt

O95971

Additional CD160 Products

Product Documents for Human CD160 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD160 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human CD160 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6700P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD160 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD160 PE-conjugated Antibody

Detection of CD160 in Human PBMCs by Flow Cytometry.