CD2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CD2 Monoclonal Antibody (Catalog # MAB18561) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CD2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human whole blood lymphocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2

CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T, NK, B, and some antigen presenting cells. Human CD2 binds human CD58 on antigen-presenting cells to induce co-stimulating signals in T cells.

Alternate Names

CD2, LFA-2, T11

Entrez Gene IDs

914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)

Gene Symbol

CD2

UniProt

P06729

Additional CD2 Products

Product Documents for Human CD2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB18561

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD2 Antibody

CD2 in Human PBMCs.