HEK293 cells transfected with Human CD20-eGFP (A) vs irrelevant-GFP (B) were stained with Human Anti-Human CD20 Monoclonal Antibody (Catalog # MAB11595) followed by Allophycocyanin-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human CD20-eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD20

Ocrelizumab is an anti-human CD20 antibody used for the treatment of advanced or relapsing multiple sclerosis. It is a humanized recombinant anti human CD20 antibody, and is used to specifically deplete B-cells. A proposed pharmacological mechanism of action is that the antibody most likely binds to the B-cell surface CD20 resulting in antibody-dependent cellular cytotoxicity and complement-mediated cell lysis, but does not impact B-cell reconstitution and preexisting humoral immunity.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11595

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD20 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

Detection of CD20 in HEK293 cells by Flow Cytometry.