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Human CD20 (Research Grade Rituximab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9575

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry

Cited:

Flow Cytometry, Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu2

Product Specifications

Immunogen

Human CD20

Specificity

Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CD20 (Research Grade Rituximab Biosimilar) Antibody

Detection of CD20 antibody in HEK293 Human cell line Transfected with Human CD20 and eGFP antibody by Flow Cytometry

Detection of CD20 in HEK293 Human cell line Transfected with Human CD20 and eGFP by Flow Cytometry

HEK293 Human cell line transfected with (A) Human CD20 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human CD20 (Rituximab Biosimilar) Monoclonal Antibody (Catalog # MAB9575) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD20 (Research Grade Rituximab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human cell line Transfected with Human CD20 and eGFP

Reviewed Applications

Read 1 review rated 5 using MAB9575 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD20

Rituximab is a biosimilar directed against the extracellular domain of CD20. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9630. The biotinylated antibody (catalog number FAB9575B) can be used in flow cytometry or various assay format to measure CD20 expression and can be detected by conjugated streptavidin.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for Human CD20 (Research Grade Rituximab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD20 (Research Grade Rituximab Biosimilar) Antibody

For research use only

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