Human CD21 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB49091
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 544411
Product Summary for Human CD21 Antibody
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD21
Ile21-Arg971
Accession # P20023
Ile21-Arg971
Accession # P20023
Specificity
Detects human CD21 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD21 Antibody
Detection of CD21 in Human Tonsil.
CD21 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human CD21 Monoclonal Antibody (Catalog # MAB49091) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CD21 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD21
CD21 (also called EBV receptor or CR2) is a 145 kDa member of the RCA (receptors of complement activation) family of proteins. It is expressed on T cells, B cells, and follicular dendritic cells. On the B cell surface, it combines with the BCR and CD19 to form a B cell activating complex. Mature human CD21 is 1013 amino acids (aa) in length. It is a type I transmembrane (TM) protein that contains a 951 aa extracellular domain (ECD) (aa 21-971) and a short 34 aa cytoplasmic tail. The ECD exhibits fifteen 60 aa SUSHI repeats. Soluble CD21 can be generated by cleavage near the TM domain. One potential splice variant shows a deletion of aa 847‑908, while another shows an insertion of 59 aa after Lys659. The ECD of human CD21 shares 71% aa identity with mouse CD21 ECD.
Alternate Names
C3DR, CD21, CR2
Gene Symbol
CR2
UniProt
Additional CD21 Products
Product Documents for Human CD21 Antibody
Product Specific Notices for Human CD21 Antibody
For research use only
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