Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9926
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu107
Product Specifications
Immunogen
Human CD25/IL-2 R alpha
Specificity
Detects Human CD25/IL-2 R alpha based on Basiliximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Basiliximab.
This
product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images
IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody.
In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Human Anti-Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926). Human IgG Isotype Control (Catalog # 1-001-A) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).
Detection of CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry.
Human PBMCs were treated with PHA (5 µg/mL for 48hr) then stained with (A) Human CD25/IL-2 R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926) or (B) Normal Human IgG control antibody (Catalog # 1-001-A), followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antiobdy (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.Applications
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
0.5 µg/106 cells
Sample: In a functional flow cytometry test, 0.5 μg/mL of Human Anti-Human CD25/IL-2R alpha Antibody(Catalog # MAB9926) will block the binding of 500 ng/mL Biotinylated Recombinant Human IL-2 (Catalog # BT202) to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha.
Sample: In a functional flow cytometry test, 0.5 μg/mL of Human Anti-Human CD25/IL-2R alpha Antibody(Catalog # MAB9926) will block the binding of 500 ng/mL Biotinylated Recombinant Human IL-2 (Catalog # BT202) to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMCs treated with PHA
Sample: Human PBMCs treated with PHA
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA
Entrez Gene IDs
Gene Symbol
IL2RA
Additional CD25/IL-2R alpha Products
Product Specific Notices
For research use only
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