Human CD34 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB72271
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 756510
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD34
Ser32-Thr290
Accession # P28906
Ser32-Thr290
Accession # P28906
Specificity
Detects human CD34 in direct ELISAs. In direct ELISAs, no cross-reactivity
with recombinant mouse, rat, or porcine CD34 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD34 Antibody
Detection of CD34 in KG-1a Human Cell Line by Flow Cytometry.
KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72271, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
CD34 in KG‑1 Human Cell Line.
CD34 was detected in immersion fixed KG-1 human acute myelogenous leukemia cell line using Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72271) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CD34 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: KG-1a acute myelogenous leukemia cell line
Sample: KG-1a acute myelogenous leukemia cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed KG‑1 human acute myelogenous leukemia cell line
Sample: Immersion fixed KG‑1 human acute myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB72271 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD34
Alternate Names
CD34, HPCA1
Gene Symbol
CD34
UniProt
Additional CD34 Products
Product Documents for Human CD34 Antibody
Product Specific Notices for Human CD34 Antibody
For research use only
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