Human CD38 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2404
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Xenograft
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 240742
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CD38
Met1-Ile300
Accession # P28907
Met1-Ile300
Accession # P28907
Specificity
Detects human CD38.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human CD38 Antibody
CD38 in Human PBMCs.
CD38 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CD38 Monoclonal Antibody (Catalog # MAB2404) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CD38 in Daudi Human Burkitt's Lymphoma Cell Line (positive) and HDLM-2 Human Hodgkin’s Lymphoma Cell Line (negative) Cells .
CD38 was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (positive) and HDLM-2 Human Hodgkin’s Lymphoma Cell Line (negative) Cells using Mouse Anti-Human CD38 Monoclonal Antibody (Catalog # MAB2404) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CD38 in human tonsil.
CD38 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD38 Monoclonal Antibody (Catalog # MAB2404) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CD38 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood lymphocytes
Sample: Human whole blood lymphocytes
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs), Daudi Human Burkitt's Lymphoma Cell Line (positive) and HDLM-2 Human Hodgkin's Lymphoma Cell Line (negative) Cells
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs), Daudi Human Burkitt's Lymphoma Cell Line (positive) and HDLM-2 Human Hodgkin's Lymphoma Cell Line (negative) Cells
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human CD38 (Catalog # 2404‑AC), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human CD38 (Catalog # 2404‑AC), see our available Western blot detection antibodies
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD38
CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells (4, 5).
References
- Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
- Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
- Sun, L. et al. (1999) J. Cell Biol. 146:1161.
- Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
- Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
Long Name
Cluster of Differentiation 38
Alternate Names
ADP-ribosyl Cyclase, CD38, Cyclic ADP-ribose Hydrolase
Gene Symbol
CD38
UniProt
Additional CD38 Products
Product Documents for Human CD38 Antibody
Product Specific Notices for Human CD38 Antibody
For research use only
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