Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD43 Monoclonal Antibody (Catalog # MAB2038) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody. View our protocol for Staining Membrane-associated Proteins.

CD43 in Human PBMCs.

CD43 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD43 Monoclonal Antibody (Catalog # MAB2038) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CD43 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2038 in the following applications:

Flow Cytometry (1 Review)
Immunoprecipitation (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD43

CD43, also known as Leukosialin and Sialophorin and Ly-48 in mouse, is a 100-105 kDa type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells. Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific. CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion. Mature CD43 shows 49% amino (aa) sequence identity between human and mouse.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Gene Symbol

SPN

UniProt

P16150

Additional CD43 Products

Product Documents for Human CD43 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD43 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD43 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2038

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD43 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD43 Antibody

Detection of CD43 in Human PBMCs by Flow Cytometry.