Human PBMC were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

CD63 in A431 Human Cell Line.

CD63 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of CD63 in PBMC monocytes by Flow Cytometry

PBMC monocytes were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD63 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human PBMCs fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012); PBMC monocytes (surface stain)

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB50481 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.

Alternate Names

CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30

Entrez Gene IDs

967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)

Gene Symbol

CD63

UniProt

P08962-1

Additional CD63 Products

Product Documents for Human CD63 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD63 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD63 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB50481

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all CD63 Antibodies »

Product Summary for Human CD63 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD63 Antibody

Detection of CD63 in Human PBMC by Flow Cytometry.