Human CD68/SR-D1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB20401
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry
Cited:
ELISA Control, IHC-, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 298807
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
Asn22-Ile320
Accession # AAB25811
Asn22-Ile320
Accession # AAB25811
Specificity
Detects human CD68/SR‑D1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD68/SR-D1 Antibody
CD68/SR-D1 in Human PBMCs.
CD68/SR-D1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Human CD68/SR-D1 Monoclonal Antibody (Catalog # MAB20401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CD68/SR-D1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol
Sample: U937 human histiocytic lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol
Reviewed Applications
Read 1 review rated 5 using MAB20401 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD68/SR-D1
Alternate Names
CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1
Gene Symbol
CD68
UniProt
Additional CD68/SR-D1 Products
Product Documents for Human CD68/SR-D1 Antibody
Product Specific Notices for Human CD68/SR-D1 Antibody
For research use only
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