Human CD7 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7579
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Kidney embryonic cell line HEK293-derived recombinant human CD7
Ala26-Pro180
Accession # P09564
Ala26-Pro180
Accession # P09564
Specificity
Detects human CD7 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CD7 Antibody
Detection of Human CD7 by Western Blot.
Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and human peripheral blood lymphocytes (PBL). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD7 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CD7 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) or (B) Sheep IgG Control (Catalog # 5-001-A) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
CD7 in Human PBMCs.
CD7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human CD7 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)
Sample: Human peripheral blood mononuclear cells (PBMCs)
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thymus
Sample: Immersion fixed paraffin-embedded sections of human thymus
Western Blot
0.5 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and human peripheral blood lymphocytes (PBL)
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and human peripheral blood lymphocytes (PBL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD7
CD7 (Cluster of Differentiation Antigen 7; also Leu-9, TP41 and GP40) is a 40-44 kDa member of the Ig-superfamily of proteins. It shows restricted expression, being found on fetal thymocytes, CD34+ myeloid and lymphoid progenitor cells, memory CLA- CD45RA+ T cells, and CD56+ IFN-gamma secreting NK cells. CD7 binds to both SECTM1/K12 and galectin-1, and when bound to the latter, initiates complex formation with CD43 in cis. Activation of CD7 may result in either cell proliferation or apoptosis, suggesting a context-dependent signaling mechanism. Mature human CD7 is a 215 amino acid (aa) type I transmembrane glycoprotein. It contains a 155 aa extracellular region (aa 26-180) that shows one V-type Ig-like domain (aa 26-130), and a 39 aa C-terminal cytoplasmic domain. There is one potential alternative splice variant that contains a 79 aa substitution for aa 133-240. Over aa 26-180, human CD7 shares only 43% aa sequence identity with mouse CD7.
Alternate Names
CD7, LEU-9, TP41
Gene Symbol
CD7
UniProt
Additional CD7 Products
Product Documents for Human CD7 Antibody
Product Specific Notices for Human CD7 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...