Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 683023

View all CD79B Antibodies »

Product Summary for Human CD79B Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD79B
Ala29-Asp159
Accession # P40259

Specificity

Detects human CD79B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD79B or recombinant human CD79A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human CD79B Antibody

Detection of CD79B antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD79B in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Human CD79B Monoclonal Antibody (Catalog # MAB6620) followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117) and Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on normal mouse IgM control antibody staining .

Applications for Human CD79B Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD79B

CD79B, also known as Igb and B29, is a 36 kDa transmembrane glycoprotein in the immunoglobulin superfamily. It contains a single Ig‑like domain in its extracellular region (ECD) and one cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). Alternate splicing generates a short isoform that lacks nearly the entire Ig‑like domain. Heterodimers of CD79A/Iga and CD79B associate with a membrane bound immunoglobulin on the B cell surface to form the B cell antigen receptor complex (BCR). CD79A and CD79B are required for BCR-mediated signaling and consequently for the development and activation of B lineage cells. Within the ECD (aa 29-159), human CD79B shares 54% aa sequence identity with mouse CD79B.

Alternate Names

B29, CD79B, IGB

Entrez Gene IDs

974 (Human); 15985 (Mouse); 171055 (Rat); 102145117 (Cynomolgus Monkey)

Gene Symbol

CD79B

UniProt

P40259

Additional CD79B Products

Product Documents for Human CD79B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD79B Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.