Mouse IgM APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # F0117
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse IgM
Specificity
Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab')2 fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IgM APC-conjugated Antibody
Detection of Mouse IgM Primary Antibody by Flow Cytometry
BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495, filled histogram) or mouse IgM isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117) at 10 µL/106cells.Applications for Mouse IgM APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of the IgM class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgM
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin M
Alternate Names
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Additional IgM Products
Product Documents for Mouse IgM APC-conjugated Antibody
Product Specific Notices for Mouse IgM APC-conjugated Antibody
For research use only
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