Human CEBP alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7094
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 692716
Product Summary for Human CEBP alpha Antibody
Immunogen
E. coli-derived recombinant human CEBP alpha
Met1-Ala124
Accession # P49715
Met1-Ala124
Accession # P49715
Specificity
Detects human CEBP alpha in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human CEBP beta, gamma, delta, epsilon, or zeta is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CEBP alpha Antibody
Detection of Human CEBP alpha by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CEBP a Monoclonal Antibody (Catalog # MAB7094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CEBP alpha in Human Mesenchymal Stem Cells.
CEBP a was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human CEBP a Monoclonal Antibody (Catalog # MAB7094) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human CEBP alpha Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human mesenchymal stem cells
Sample: Immersion fixed human mesenchymal stem cells
Western Blot
1 µg/mL
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB7094 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEBP alpha
Long Name
CCAAT/enhancer-Binding Protein alpha
Alternate Names
C/EBP-alpha, CEBPA
Gene Symbol
CEBPA
UniProt
Additional CEBP alpha Products
Product Documents for Human CEBP alpha Antibody
Product Specific Notices for Human CEBP alpha Antibody
For research use only
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