Human CHD7 Antibody

CHD7 (Chromohelicase/ATPase DNA-binding protein 7) is a 350 kDa member of the third subgroup in the SNF2/RAD54 helicase family of proteins. It is an ATP‑dependent chromatin remodeling factor that also binds histones (H3 at Lys4) and influences transcription. CHD7 binds more that 10,000 sites on chromatin, particularly in locations associated with gene enhancement. Notably, CHD7 is most associated with the nervous system, and is found in embryonic hypothalamus (GnRH neurons), olfactory epithelium and spinal cord, and adult preoptic hypothalamus plus hippocampus. Human CHD7 is 2997 amino acids (aa) in length. It contains consecutive Gln-, Pro- and Lys-rich regions (aa 151-718), two Chromo (chromatin-organizer-modifer) domains (aa 800-947), a helicase ATP-binding domain (aa 980-1154), a C-terminal helicase domain (aa 1294-1464), one coiled-coil region (aa 2410-2431) and two BRK domains (aa 2562-2604 and 2642-2686). There are at least seven utilized Ser/Thr phosphorylation sites, and three potential isoform variants. One isoform contains a 12 aa substitution for aa 1127-2997, a second 145 kDa isoform shows a deletion of aa 573-2621, while a third isoform contains a 15 aa insertion after Ser698. CHD7 is reported to bind to subgroup three member CHD8. Over aa 263-457, human CHD7 shares 96% aa identity with mouse CHD7.