Detects human Chordin-like 2/CHRDL2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse CHRDL1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Chordin-like 2/CHRDL2 Antibody

Detection of Chordin-like 2/CHRDL2 in MCF-7 cells by Flow Cytometry.

MCF-7 cells were stained with Mouse Anti-Human Chordin-like 2/CHRDL2 Monoclonal Antibody (Catalog # MAB24481, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human Chordin-like 2/CHRDL2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: MCF‑7 human breast cancer cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Western Blot

1 µg/mL
Sample: Recombinant Human Chordin-like 2/CHRDL2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

CHRDL2, also known as breast tumor novel factor 1 (BNF-1), is a secreted BMP antagonist implicated in chondrocyte development.

Alternate Names

BNF-1, Chordin like 2, CHRDL2

Entrez Gene IDs

25884 (Human); 69121 (Mouse)

Gene Symbol

CHRDL2

UniProt

NP_056239

Additional Chordin-like 2/CHRDL2 Products

Product Documents for Human Chordin-like 2/CHRDL2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Chordin-like 2/CHRDL2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Chordin-like 2/CHRDL2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB24481

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Chordin-like 2/CHRDL2 Antibodies »

Product Specifications

Immunogen

Specificity