HEK293 human embryonic kidney cell line transfected with (A) human CHRM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

CHRM3 in Human Brain (Cortex) Tissue.

CHRM3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) tissue using Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CHRM3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human CHRM3 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM3

Muscarinic acetylcholine receptor M3 (CHRM3) is a 65 kDa 7-transmembrane glycoprotein that is predominantly expressed in the central nervous system, smooth muscle, and heart. CHRM3 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Human CHRM3 shares 92% aa sequence identity with mouse and rat CHRM3.

Long Name

Cholinergic Receptor Muscarinic 3

Alternate Names

HM3, mAChR M3

Entrez Gene IDs

1131 (Human); 12671 (Mouse); 24260 (Rat)

Gene Symbol

CHRM3

UniProt

P20309

Additional CHRM3 Products

Product Documents for Human CHRM3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CHRM3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CHRM3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6378

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CHRM3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CHRM3 Antibody

Detection of CHRM3 in HEK293 Human Cell Line Transfected with Human CHRM3 and eGFP by Flow Cytometry.