Human Chromogranin A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB90982
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 948130
Product Summary for Human Chromogranin A Antibody
Immunogen
Human embryonic kidney cell line HEK293-derived human Chromogranin A
Met1-Gly457
Accession # P10645
Met1-Gly457
Accession # P10645
Specificity
Detects human Chromogranin A in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Chromogranin A Antibody
Human Chromogranin A ELISA Standard Curve.
Recombinant Human Chromogranin A protein was serially diluted 2-fold and captured by Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB9098) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90982) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Chromogranin A Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB9098).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Chromogranin A DuoSet ELISA Kit (Catalog # DY9098-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chromogranin A
Alternate Names
Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I
Gene Symbol
CHGA
UniProt
Additional Chromogranin A Products
Product Documents for Human Chromogranin A Antibody
Product Specific Notices for Human Chromogranin A Antibody
For research use only
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