Human CLCA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10766
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1022517
Product Specifications
Immunogen
Human embryonic kidney cell HEK293-derived CLCA1
Asn22-Ser477
Accession # A8K714
Asn22-Ser477
Accession # A8K714
Specificity
Detects CLCA1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CLCA1 Antibody
CCL1/I-309/TCA-3 in Human Colon.
CCL1/I-309/TCA-3 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL1/I-309/TCA-3 Monoclonal Antibody (Catalog # MAB10766) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Goblet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human CLCA1 by Simple WesternTM.
Simple Western lane view shows lysates of human small intestine, loaded at 0.2 mg/mL. Specific bands were detected for CLCA1 at approximately 55 and 89 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CLCA1 Monoclonal Antibody (Catalog # MAB10766) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human CLCA1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Simple Western
20 µg/mL
Sample: Human small intestine
Sample: Human small intestine
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLCA1
Long Name
Chloride Channel Accessory 1
Alternate Names
CaCC-1, CLCRG1, GOB5, HCLCA1
Gene Symbol
CLCA1
UniProt
Additional CLCA1 Products
Product Documents for Human CLCA1 Antibody
Product Specific Notices for Human CLCA1 Antibody
For research use only
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