Human CLEC10A/CD301 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB48881R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 744812
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301
Gln61-His316
Accession # Q8IUN9
Gln61-His316
Accession # Q8IUN9
Specificity
Detects human CLEC10A/CD301 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human ASGPR1 or recombinant mouse MGL1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human CLEC10A/CD301 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Immature human dendritic cells
Sample: Immature human dendritic cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CLEC10A/CD301
CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64%-70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha- or beta-linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).
References
- Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
- Suzuki, N. et al. (1996) J. Immunol. 156:128.
- Higashi, N. et al. (2002) J. Biol. Chem. 277:20686.
- Valladeau, J. et al. (2001) J. Immunol. 167:5767.
- van Vliet, S.J. et al. (2006) Immunobiology 211:577.
- van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
- Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
- van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
- Higashi, N. et al. (2002) Int. Immunol. 14:545.
- Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
- Takada, A. et al. (2004) J. Virol. 78:2943.
Long Name
Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1
Alternate Names
CD301, CLECSF14, HML2, MGL
Entrez Gene IDs
10462 (Human)
Gene Symbol
CLEC10A
UniProt
Additional CLEC10A/CD301 Products
Product Documents for Human CLEC10A/CD301 Alexa Fluor® 647-conjugated Antibody
Product Specific Notices for Human CLEC10A/CD301 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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