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Human CLEC10A/CD301 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF4888

R&D Systems, part of Bio-Techne
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BAF4888

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Detection

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301 (Catalog # 4888-CL)
Gln61-His316
Accession # Q8IUN9

Specificity

Detects human CLEC10A/CD301 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) CLEC1, rhCLEC2, and rhCLEC3B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CLEC10A/CD301 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CLEC10A/CD301 (Catalog # 4888-CL)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64% - 70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is up-regulated by the immunosuppressant dexamethasone (3-7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha- or beta-linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).

References

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Suzuki, N. et al. (1996) J. Immunol. 156:128. 
  3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686. 
  4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.
  5. van Vliet, S.J. et al. (2006) Immunobiology 211:577. 
  6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
  7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
  8. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
  9. Higashi, N. et al. (2002) Int. Immunol. 14:545.
  10. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
  11. Takada, A. et al. (2004) J. Virol. 78:2943.

Long Name

Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1

Alternate Names

CD301, CLECSF14, HML2, MGL

Entrez Gene IDs

10462 (Human)

Gene Symbol

CLEC10A

UniProt

Additional CLEC10A/CD301 Products

Product Documents for Human CLEC10A/CD301 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CLEC10A/CD301 Biotinylated Antibody

For research use only

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