Human CNTFR alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB303
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 121723
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha
Gln23-Pro346 (predicted)
Accession # P26992
Gln23-Pro346 (predicted)
Accession # P26992
Specificity
Detects human CNTF R alpha in direct ELISAs and Western blots. In Western blots, approximately 100% cross-reactivity with recombinant rat CNTF R alpha is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CNTFR alpha Antibody
Detection of CNTFR alpha in Human Skeletal Muscle.
CNTFR alpha was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human CNTFR alpha Monoclonal Antibody (Catalog # MAB303) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma and sarcoplasm of skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CNTFR alpha Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Skeletal Muscle
Sample: Immersion fixed paraffin-embedded sections of Human Skeletal Muscle
Western Blot
1 µg/mL
Sample: Recombinant Human CNTF R alpha (Catalog # 303-CR)
Sample: Recombinant Human CNTF R alpha (Catalog # 303-CR)
Reviewed Applications
Read 1 review rated 5 using MAB303 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTFR alpha
Long Name
Ciliary Neurotrophic Factor Receptor alpha
Alternate Names
CNTF R alpha, CNTFR, CNTFRa
Gene Symbol
CNTFR
UniProt
Additional CNTFR alpha Products
Product Documents for Human CNTFR alpha Antibody
Product Specific Notices for Human CNTFR alpha Antibody
For research use only
Loading...
Loading...
Loading...
Loading...