Human CNTFR alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # AF-303-NA

R&D Systems, part of Bio-Techne
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AF-303-SP
AF-303-NA
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Conjugate
Catalog #
Biotin
BAF303
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Feline

Applications

Validated:

Neutralization, Western Blot

Cited:

Flow Cytometry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CNTFR alpha Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha
Gln23-Pro346 (predicted)
Accession # P26992

Specificity

Detects human CNTF R alpha in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CNTFR alpha Antibody

CNTF R alpha Enhancement of CNTF-dependent Cell Proliferation and Neutral-ization by Human CNTF R alpha Antibody.

CNTF R alpha Enhancement of CNTF-dependent Cell Proliferation and Neutral-ization by Human CNTF R alpha Antibody.

Recombinant Human CNTF Ra (Catalog # 303-CR) enhances Recom-binant Human CNTF (Catalog # 257-NT) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recom-binant Human CNTF Ra (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Ra Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF-303-NA). The ND50 is typically 10-20 µg/mL.

Applications for Human CNTFR alpha Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CNTF R alpha (Catalog # 303-CR)

Neutralization

Measured by its ability to neutralize CNTF R alpha-enhanced proliferation in the TF-1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 1 µg/mL Recombinant Human CNTF R alpha and 20 ng/mL Recombinant Human CNTF.

Reviewed Applications

Read 1 review rated 4 using AF-303-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTFR alpha

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha subunit (CNTF R alpha), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha (CNTF sR alpha) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha.

Long Name

Ciliary Neurotrophic Factor Receptor alpha

Alternate Names

CNTF R alpha, CNTFR, CNTFRa

Entrez Gene IDs

1271 (Human); 12804 (Mouse); 313173 (Rat)

Gene Symbol

CNTFR

UniProt

P26992

Additional CNTFR alpha Products

Product Documents for Human CNTFR alpha Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CNTFR alpha Antibody

For research use only

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