Human CNTFR alpha Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF303

R&D Systems, part of Bio-Techne
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BAF303
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all CNTFR alpha Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha

Specificity

Detects human CNTF R alpha in Western blots. In Western blots (under non-reducing and reducing conditions), greater than 50% cross‑reactivity with recombinant rat CNTF sR alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CNTFR alpha Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CNTF R alpha (Catalog # 303-CR)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTFR alpha

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha subunit (CNTF R alpha), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha (CNTF sR alpha) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha.

Long Name

Ciliary Neurotrophic Factor Receptor alpha

Alternate Names

CNTF R alpha, CNTFR, CNTFRa

Entrez Gene IDs

1271 (Human); 12804 (Mouse); 313173 (Rat)

Gene Symbol

CNTFR

Additional CNTFR alpha Products

Product Documents for Human CNTFR alpha Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human CNTFR alpha Biotinylated Antibody

For research use only

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