Human Collagen IV alpha1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6308
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry
Cited:
ELISA Capture
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 577238
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Collagen IV alpha1
Ser1441-Thr1669
Accession # P02462
Ser1441-Thr1669
Accession # P02462
Specificity
Detects human Collagen IV alpha1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Collagen I alpha 1, III alpha 1, XIII alpha 1v4, XXIII alpha 1, or recombinant mouse Collagen XXV alpha 1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Collagen IV alpha1 Antibody
Collagen IV alpha1 in T98G Human Cell Line.
Collagen IV a1 was detected in immersion fixed T98G human glioblastoma cell line using Mouse Anti-Human Collagen IV alpha 1 Monoclonal Antibody (Catalog # MAB6308) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to perinuclear areas and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Collagen IV alpha1 in THP‑1 Human Cell Line.
Collagen IV a1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Collagen IV a1 Monoclonal Antibody (Catalog # MAB6308) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Collagen IV alpha1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed T98G human glioblastoma cell line and THP-1 human acute monocytic leukemia cell line
Sample: Immersion fixed T98G human glioblastoma cell line and THP-1 human acute monocytic leukemia cell line
Reviewed Applications
Read 1 review rated 5 using MAB6308 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen IV alpha 1
COL4A1 (collagen IV alpha1) is a 185 kDa member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells. Two COL4A1 molecules interact with a 170 kDa alpha2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642 amino acids (aa) in length. It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669). Multiple splice forms exist. One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669. Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.
Alternate Names
Arresten, COL4A1
Gene Symbol
COL4A1
UniProt
Additional Collagen IV alpha 1 Products
Product Documents for Human Collagen IV alpha1 Antibody
Product Specific Notices for Human Collagen IV alpha1 Antibody
For research use only
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