Human Complement Factor H-related 4/
CFHR4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5980
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Detection
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4
Arg41-Glu331 (Ser79Leu)
Accession # Q92496
Arg41-Glu331 (Ser79Leu)
Accession # Q92496
Specificity
Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) CFHR1, rhCFHR2, and rhCFHR5 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Complement Factor H-related 4/CFHR4 Antibody
Detection of Human Complement Factor H-related 4/CFHR4 by Western Blot.
Western blot shows lysates of human plasma. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Complement Factor H-related 4/CFHR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5980) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor H-related 4/CFHR4 at approximately 86 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Complement Factor H-related 4/CFHR4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human plasma
Sample: Human plasma
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor H-related 4/CFHR4
FHR4 (Complement factor H-related protein 4; also FHR4B and CFHR4) is a 45 kDa glycoprotein member of the factor H-related protein family. It is synthesized by hepatocytes and circulates in plasma. FHR4(B) binds to C3b and C3d. It also binds to native (pentameric) C-reactive protein (pCRP), and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance. Mature human CFHR is 312 amino acids (aa) in length. It contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. There is a circulating, 86 kDa alternative splice form termed FHR4A that shows an insertion of 247 aa after Asp92. This is considered the dominant isoform, and appears to show the same activity as CFHR. Over aa 41-331, human CFHR shares 61% aa identity with a related molecule found in rat.
Alternate Names
CFHL4, CFHR4, Complement Factor Hrelated 4, FHR4
Entrez Gene IDs
10877 (Human)
Gene Symbol
CFHR4
UniProt
Additional Complement Factor H-related 4/CFHR4 Products
- All Products for Complement Factor H-related 4/CFHR4
- Complement Factor H-related 4/CFHR4 cDNA Clones
- Complement Factor H-related 4/CFHR4 ELISA Kits
- Complement Factor H-related 4/CFHR4 Lysates
- Complement Factor H-related 4/CFHR4 Primary Antibodies
- Complement Factor H-related 4/CFHR4 Proteins and Enzymes
Product Documents for Human Complement Factor H-related 4/CFHR4 Antibody
Product Specific Notices for Human Complement Factor H-related 4/CFHR4 Antibody
For research use only
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