Human CPT1A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11570

R&D Systems, part of Bio-Techne
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MAB11570-100
MAB11570-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1081618

View all CPT1A Antibodies »

Product Specifications

Specificity

Detects a synthetic peptide corresponding to residues surrounding amino acid 501 of human CPT1A protein in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CPT1A Antibody

Detection of CPT1A in Human Kidney.

CPT1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CPT1A Monoclonal Antibody (Catalog # MAB11570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CPT1A Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Formulation, Preparation, and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CPT1A

CPT1A is one of the isoforms of CPT1.  CPT1 is a mitochondrial enzyme responsible for the formation of acyl carnitines. CPT1A is the rate-limiting enzyme in the fatty acid beta-oxidation, allowing fatty acids to enter the mitochondrial matrix for oxidation. Deficiency or abnormal regulation in this process can result in diseases like metabolic disorders and cancer including but not limited to: CPT1A deficiency, metabolic syndrome, obesity, type 2 diabetes, vascular disease, heart failure, non-alcoholic fatty liver disease, MS, and renal fibrosis. CPT1A is expressed on the outer mitochondrial membrane of most tissues but predominates in lipogenic tissues such as liver. Genetic, physiological, and dietary modulators are all involved in the regulation of CPT1A. 

References

  1. Brown NF, Hill JK, Esser V, Kirkland JL, Corkey BE, Foster DW, McGarry JD. Mouse white adipocytes and 3T3-L1 cells display an anomalous pattern of carnitine palmitoyltransferase (CPT) I isoform expression during differentiation. Inter-tissue and inter-species expression of CPT I and CPT II enzymes. Biochem J. 1997 Oct 1;327 ( Pt 1)(Pt 1):225-31. doi: 10.1042/bj3270225. PMID: 9355756; PMCID: PMC1218784.
  2. Liang K. Mitochondrial CPT1A: Insights into structure, function, and basis for drug development. Front Pharmacol. 2023 Mar 23;14:1160440. doi: 10.3389/fphar.2023.1160440. PMID: 37033619; PMCID: PMC10076611.

Long Name

Carnitine O-palmitoyltransferase 1, liver isoform

Alternate Names

CPT I, CPT1, CPT1-L, CPTI-L, EC 2.3.1.21

Entrez Gene IDs

1374 (Human); 12894 (Mouse); 25757 (Rat)

Gene Symbol

CPT1A

UniProt

P50416 (Human)

Additional CPT1A Products

Product Documents for Human CPT1A Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CPT1A Antibody

For research use only

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