Human CPT1A Antibody

Name: Human CPT1A Antibody
Brand: R&D Systems
SKU: MAB11570

R&D Systems, part of Bio-Techne | Catalog # MAB11570

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Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Human
Applications:	Immunohistochemistry
Label:	Unconjugated
Specificity:	Detects a synthetic peptide corresponding to residues surrounding amino acid 501 of human CPT1A protein in direct ELISA.

View Full Technical Details for Human CPT1A Antibody

Technical Data
Citations / Reviews

Applications for Human CPT1A Antibody

	Recommended Concentration	Sample
Immunohistochemistry	3-25 µg/mL	Immersion fixed paraffin-embedded sections of human kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CPT1A Antibody

	Immunohistochemistry Detection of CPT1A in Human Kidney. CPT1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CPT1A Monoclonal Antibody (Catalog # MAB11570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Stability & Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CPT1A

CPT1A is one of the isoforms of CPT1. CPT1 is a mitochondrial enzyme responsible for the formation of acyl carnitines. CPT1A is the rate-limiting enzyme in the fatty acid beta -oxidation, allowing fatty acids to enter the mitochondrial matrix for oxidation. Deficiency or abnormal regulation in this process can result in diseases like metabolic disorders and cancer including but not limited to: CPT1A deficiency, metabolic syndrome, obesity, type 2 diabetes, vascular disease, heart failure, non‑alcoholic fatty liver disease, MS, and renal fibrosis. CPT1A is expressed on the outer mitochondrial membrane of most tissues but predominates in lipogenic tissues such as liver. Genetic, physiological, and dietary modulators are all involved in the regulation of CPT1A.

Item	Value
References	Brown NF, Hill JK, Esser V, Kirkland JL, Corkey BE, Foster DW, McGarry JD. Mouse white adipocytes and 3T3-L1 cells display an anomalous pattern of carnitine palmitoyltransferase (CPT) I isoform expression during differentiation. Inter-tissue and inter-species expression of CPT I and CPT II enzymes. Biochem J. 1997 Oct 1;327 ( Pt 1)(Pt 1):225-31. doi: 10.1042/bj3270225. PMID: 9355756; PMCID: PMC1218784. Liang K. Mitochondrial CPT1A: Insights into structure, function, and basis for drug development. Front Pharmacol. 2023 Mar 23;14:1160440. doi: 10.3389/fphar.2023.1160440. PMID: 37033619; PMCID: PMC10076611.
Long Name	Carnitine O-palmitoyltransferase 1, liver isoform
Entrez Gene IDs	1374 (Human); 12894 (Mouse); 25757 (Rat)
Alternate Names	CPT I, CPT1, CPT1-L, CPT1-LEC 2.3.1.21, CPT1A, CPTI-L, Carnitine O-palmitoyltransferase I, liver isoform, Carnitine palmitoyltransferase 1A, EC 2.3.1, EC 2.3.1.21, L-CPT1, carnitine O-palmitoyltransferase 1, liver isoform, carnitine palmitoyltransferase 1A (liver), carnitine palmitoyltransferase I, liver

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Human CPT1A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11570

Key Product Details

Applications for Human CPT1A Antibody

Scientific Data Examples for Human CPT1A Antibody

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CPT1A

Customer Reviews for Human CPT1A Antibody

Product Documents for Human CPT1A Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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