Human CTGF/CCN2 C-Terminus Antibody

Detection of Human CTGF/CCN2 by Immunocytochemistry/ Immunofluorescence

Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human CTGF/CCN2 by Western Blot

Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta-actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta-actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P <0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&D Systems.