Human CXCR1/IL-8RA Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB330

Clone 42705 was used by HLDA to establish CD designation
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Guinea Pig, Rabbit

Applications

Validated:

CyTOF-reported, Flow Cytometry, Neutralization

Cited:

Bioassay, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 42705

View all CXCR1/IL-8RA Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
Met1-Leu350
Accession # AAA59159

Specificity

Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCR1/IL-8RA Antibody

Detection of CXCR1/IL-8 RA antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody.

Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody.

Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL-8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330). The ND50 is typically 0.4-2.0 µg/mL.

Applications for Human CXCR1/IL-8RA Antibody

Application
Recommended Usage

CyTOF-reported

Cheng, Y. et al. (2016) J. Immunol. 196: 924. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood granulocytes

Neutralization

Measured by its ability to neutralize CXCL8/IL-8-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR1. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human CXCL8/IL-8.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB330 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR1/IL-8RA

The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

Long Name

Interleukin 8 Receptor A

Alternate Names

CD181, IL-8 RA, IL8RA

Entrez Gene IDs

3577 (Human); 227288 (Mouse); 54258 (Rat)

Gene Symbol

CXCR1

UniProt

AAA59159

Additional CXCR1/IL-8RA Products

Product Documents for Human CXCR1/IL-8RA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CXCR1/IL-8RA Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.