Human CXCR3 Antibody

Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCR3 Antibody.

Recombinant Human CXCL11/I-TAC (672-IT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL11/I-TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160). The ND₅₀ is typically 0.3-1.5 µg/mL.

Detection of Human CXCR3 by Western Blot

Functional effects of IP-10 on PCCs(A) Expression of CXCR3 in PCCs, PSCs and HEK293-EBNA/CXCR3-B analyzed by Western Blot. alpha-tubulin was used as loading control. (B) Microphotographs of PCCs migrated through the Boyden chamber in response to 0.5% FBS or IP-10 (24 hr). (C-D) Viability of PCCs after IP-10 addition determined using the MTT assay. Low/high refers to cell seeding density. Data are representative of two/three independent experiments. Data in the histograms are presented as mean ± SD. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.2519), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human CXCR3 by Immunocytochemistry/Immunofluorescence

Infiltration of CXCR3+ cells in the lamina propria of small intestine mucosa.a. Confocal immunofluorescence for CXCR3 was performed in sections of duodenal biopsies from controls (i), untreated celiac patients (ii), and treated celiac patients (iii). CXCR3 is shown in green, and nuclei are shown in red. Untreated celiac patients showed a higher number of positive cells infiltrating the LP. (Magnification, 630×). b. The number of CXCR3+ cells in the LP was higher in the duodenal mucosa of untreated celiac patients (n = 9) compared with control individuals (n = 6) and treated patients (n = 5) (unpaired t-test; p = 0.0089 and p = 0.0055, respectively). The positive cells in LP regions from sections of duodenal biopsies were counted using immunofluorescence microscopy. c. Representative flow cytometric analysis from the LP compartment of a duodenal sample of an untreated CD patient showing plasma cells (CD138+) that express CXCR3. d. Representative flow cytometric analysis from the LP compartment of a duodenal sample of an untreated CD patient showing LP lymphocytes (CD3+ or CD4+) that express CXCR3. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0089068), licensed under a CC-BY license. Not internally tested by R&D Systems.