Human CYP7A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9120

R&D Systems, part of Bio-Techne
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MAB9120-100
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 946902

View all CYP7A1 Antibodies »

Product Specifications

Immunogen

Human CYP7A1 synthetic peptide
Accession # P22680

Specificity

Detects human CYP7A1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CYP7A1 Antibody

CYP7A1 antibody in Human Hepatocytes by Immunocytochemistry (ICC).

CYP7A1 in Human Hepatocytes.

CYP7A1 was detected in immersion fixed human hepatocytes induced with dexamethasone for 48 hours using Mouse Anti-Human CYP7A1 Polyclonal Antibody (Catalog # MAB9120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (green). Specific staining was localized to endoplasmic reticula and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CYP7A1 Antibody

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample:

Immersion fixed human hepatocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CYP7A1

CYP7A1 (cytochrome P450 7A1; also Cholesterol 7-alph-monooxygenase) is a 504 aa oxidoreductase enzyme (SwissProt #: P22680).  Human CYP7A1 shares 81% and 82% aa identity with mouse and rat CYP7A1, respectively.  It is expressed in the liver where it catalyzes cholesterol metabolism, which is the rate-limiting step in bile acid synthesis.  Disruption of CYP7A1 is associated with high plasma cholesterol levels leading to increased risk of coronary artery disease.

Long Name

Cytochrome P450 7A1

Alternate Names

CP7A, CYP7, CYPVII

Entrez Gene IDs

1581 (Human); 13122 (Mouse); 25428 (Rat)

Gene Symbol

CYP7A1

UniProt

P22680

Additional CYP7A1 Products

Product Documents for Human CYP7A1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CYP7A1 Antibody

For research use only

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