Human Cytokeratin 19 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3506
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cytokeratin 19
Thr2-Leu400
Accession # P08727
Thr2-Leu400
Accession # P08727
Specificity
Detects human Cytokeratin 19 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human Cytokeratin 14 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Cytokeratin 19 Antibody
Detection of Human Cytokeratin 19 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, DU145 human prostate carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytokeratin 19 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytokeratin 19 in MCF‑7 Human Cell Line.
Cytokeratin 19 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cytokeratin 19 in Human Kidney.
Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cytokeratin 19 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Simple Western
1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line
Sample: HT‑29 human colon adenocarcinoma cell line
Western Blot
0.1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line, DU145 human prostate carcinoma cell line, HCT‑116 human colorectal carcinoma cell line, and MCF‑7 human breast cancer cell line
Sample: HT‑29 human colon adenocarcinoma cell line, DU145 human prostate carcinoma cell line, HCT‑116 human colorectal carcinoma cell line, and MCF‑7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 19
Cytokeratin 19 (Keratin, type I cytoskeletal 19; also KRT-19, CK19 and Keratin-19) is a 40-45 kDa, acidic Class I keratin member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I KRT-19 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 (plus KRT-5 and -7) to form 8-10 nm filaments in epidermal stem cells, secretory gland (sweat; mammary; bile duct) simple epithelium, and neuroendocrine epidermal Merkel cells. It may represent a viable marker for skin stem cells. In skin, Cytokeratin 19 forms filaments in the fetal epithelium, and then progressively decreases with age, being virtually absent by age 17. Human Cytokeratin 19 is 400 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79) and a subsequent "rod" region (aa 80-387), but is absent a typical C-terminal tail region. Cytokeratin 19 possesses at least 5 utilized phosphorylation sites plus one acetylated Lys residue. Based on other keratins, and the presence of an Asp at position 238, there may be caspase cleavage-generated isoforms. Full length human Cytokeratin 19 (aa 2-400) shares 82% aa sequence identity with mouse Cytokeratin 19.
Alternate Names
CK19, EndoC, K19, Krt19
Gene Symbol
KRT19
UniProt
Additional Cytokeratin 19 Products
Product Documents for Human Cytokeratin 19 Antibody
Product Specific Notices for Human Cytokeratin 19 Antibody
For research use only
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