Human Cytosolic beta-Glucosidase/GBA3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB59691

R&D Systems, part of Bio-Techne
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MAB59691
MAB59691-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 728702

View all Cytosolic beta-Glucosidase/GBA3 Antibodies »

Product Summary for Human Cytosolic beta-Glucosidase/GBA3 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosolic beta‑Glucosidase/GBA3
Thr13-Leu469
Accession # Q9H227

Specificity

Detects human Cytosolic beta‑Glucosidase/GBA3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Cytosolic beta-Glucosidase/GBA3 Antibody

Cytosolic beta-Glucosidase/GBA3 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Cytosolic beta‑Glucosidase/GBA3 in HeLa Human Cell Line.

Cytosolic beta-Glucosidase/GBA3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Cytosolic beta-Glucosidase/GBA3 Monoclonal Antibody (Catalog # MAB59691) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Cytosolic beta-Glucosidase/GBA3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic beta-Glucosidase/GBA3

There are three beta-glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide (1). GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides (2). GBA3 is a cytosolic beta-glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiological beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (3). GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (4). At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process (3, 4).

References

  1. Tybulewicz, V.L. et al. (1992) Nature 357:407.
  2. Matern, H. et al. (2001) J. Biol. Chem. 276:37929.
  3. de Graaf, M. et al. (2001) Biochem. J. 356:907.
  4. Hayashi, Y. et al. (2007) J. Biol. Chem. 282:30889.

Alternate Names

CBGL1, Cytosolic betaGlucosidase, GBA3, GLUC, KLrP

Entrez Gene IDs

57733 (Human); 289687 (Rat)

Gene Symbol

GBA3

UniProt

Q9H227 (Human)

Additional Cytosolic beta-Glucosidase/GBA3 Products

Product Documents for Human Cytosolic beta-Glucosidase/GBA3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Cytosolic beta-Glucosidase/GBA3 Antibody

For research use only

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