Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7458

R&D Systems, part of Bio-Techne
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AF7458
AF7458-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Cytosolic Sulfotransferase 1C2/SULT1C2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1C2/SULT1C2
Ala2-Leu296, predicted
Accession # O00338

Specificity

Detects human Cytosolic Sulfotransferase 1C2/SULT1C2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SULT1C4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody

Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 antibody by Western Blot.

Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 by Western Blot.

Western blot shows lysates of Caki-2 human clear cell carcinoma epithelial cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cytosolic Sulfotransferase 1C2/ SULT1C2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7458) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 1C2/SULT1C2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Caki‑2 human clear cell carcinoma epithelial cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 1C2/SULT1C2

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi-resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1C2 is mainly expressed in the gastrointestinal tract (stomach, duodenum, jejunum, ileum, colon, caecum and rectum), liver and kidneys, but not in the lungs (3). In contrast, SULT1C4, a sulfotransferase that is most closely related to SULT1C2 (4), is expressed at higher levels in fetal lung and kidney and at lower levels in fetal heart. So far, SULT1C2 is found to be active only on p-nitrophenol (3). The enzymatic activity of our recombinant human SULT1C2 was determined using a phosphatase-coupled assay (5).

References

  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Hehonah, N. et al. (1999) Int J. Biochem. Cell. Biol. 31:869.
  4. Sakakibara, Y. et al. (1998) J. Biol. Chem. 273:33929.
  5. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

ST1C1, ST1C2, SULT1C1, SULT1C2

Entrez Gene IDs

6819 (Human); 316153 (Rat)

Gene Symbol

SULT1C2

UniProt

O00338 (Human)

Additional Cytosolic Sulfotransferase 1C2/SULT1C2 Products

Product Documents for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody

For research use only

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