Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5828
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 2A1/SULT2A1
Ser2-Glu285
Accession # Q06520
Ser2-Glu285
Accession # Q06520
Specificity
Detects human Cytosolic Sulfotransferase 2A1/SULT2A1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, and rhSULT4A1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
Detection of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 by Western Blot.
Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5828) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Cytosolic Sulfotransferase 2A1/SULT2A1 in HepG2 Human Cell Line.
Cytosolic Sulfotransferase 2A1/SULT2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
0.5 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Neutralization
Measured by its ability to neutralize Recombinant Human Cytosolic Sulfotransferase 2A1/SULT2A1 (8.33 µg/mL, Catalog # 5828-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 33 μM) to Trans-Dehydroandrosterone (DHEA, 133 µM). The Neutralization Dose (ND50) is typically 35.9 µg/mL.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic Sulfotransferase 2A1/SULT2A1
Sulfotransferase 2A1 (SULT2A1), also called DHEA-ST (DHEA/bile acid sulfotransferase), is a 35 kDa member of the cytosolic sulfotransferase family, which are Phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. SULT2A1 is primarily expressed in liver and adrenal tissues. It sulfates steroids and bile acids, playing roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and preventing toxicity from bile acid accumulation. Human SULT2A1 shares 63-65% amino acid identity with mouse and rat SULT2A1.
Alternate Names
DHEA-ST, DHEAS, hSTa, ST2A3, SULT2A1
Gene Symbol
SULT2A1
UniProt
Additional Cytosolic Sulfotransferase 2A1/SULT2A1 Products
Product Documents for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
Product Specific Notices for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
For research use only
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