Human DAP12 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB52401
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 406209
Product Specifications
Immunogen
Human Dap12 synthetic peptide
Accession # O43914
Accession # O43914
Specificity
Detects human DAP12 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human DAP12 Antibody
Detection of DAP12 in Human CD56+Natural Killer Cells by Flow Cytometry.
Human PBMCs were stained with (A) Human DAP12 Monoclonal Antibody (Catalog # MAB52401) or (B) isotype control antibody (MAB003), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B) and Rabbit anti-human CD56 PE-conjugated Monoclonal Antibody (FAB24086P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (1X) (FC005).Applications for Human DAP12 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/mL
Sample: Human PBMCs
Sample: Human PBMCs
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DAP12
DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.
Long Name
DNAX-activation Protein 12
Alternate Names
KARAP, TYROBP
Gene Symbol
TYROBP
UniProt
Additional DAP12 Products
Product Documents for Human DAP12 Antibody
Product Specific Notices for Human DAP12 Antibody
For research use only
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