Human Dishevelled-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3754
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Dishevelled-3
His545-Gly654
Accession # Q92997
His545-Gly654
Accession # Q92997
Specificity
Detects human Dishevelled-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Dishevelled-1 and recombinant human Dishevelled-2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Dishevelled-3 Antibody
Detection of Human and Mouse Dishevelled-3 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dishevelled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3754) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dishevelled-3 at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Dishevelled-3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dishevelled-3
Dishevelled-3 is one of three mammalian multi-moduled Dishevelled proteins that have important roles in the canonical and non-canonical Wnt pathways. Human Dishevelled-3 is a 716 amino acid residue protein with an N-terminal DIX domain, a central PZD domain, and a DEP domain. Over the region used as immunogen, human Dishevelled-3 shares 99% amino acid sequence homology with mouse and canine Dishevelled-3.
Alternate Names
Dishevelled3, DVL3
Gene Symbol
DVL3
UniProt
Additional Dishevelled-3 Products
Product Documents for Human Dishevelled-3 Antibody
Product Specific Notices for Human Dishevelled-3 Antibody
For research use only
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