Human DNMT3A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6315
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DNMT3A
Ala353-Lys486
Accession # Q9Y6K1
Ala353-Lys486
Accession # Q9Y6K1
Specificity
Detects human DNMT3A in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human DNMT1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DNMT3A Antibody
DNMT3A in HeLa Human Cell Line.
DNMT3A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human DNMT3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6315) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DNMT3A in HeLa Human Cell Line.
DNMT3A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Goat Anti-Human DNMT3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6315) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human DNMT3A Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed OVCAR‑3 human ovarian carcinoma cell line and immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed OVCAR‑3 human ovarian carcinoma cell line and immersion fixed HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DNMT3A
Long Name
DNA [Cytosine-5-]-Methyltransferase 3A
Alternate Names
DNA (cytosine-5-)-methyltransferase 3 alpha, DNA (cytosine-5)-methyltransferase 3A, DNA cytosine methyltransferase 3A2, DNA Methyltransferase 3 Alpha, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, Dnmt3a, DNMT3A2, EC 2.1.1.37, M.HsaIIIA, TBRS
Gene Symbol
DNMT3A
UniProt
Additional DNMT3A Products
Product Documents for Human DNMT3A Antibody
Product Specific Notices for Human DNMT3A Antibody
For research use only
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