Human DNMT3B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7646
Discontinued Product
MAB7646 has been discontinued.
View all DNMT3B products.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 832121
Product Specifications
Immunogen
E. coli-derived recombinant human DNMT3B
Met1-Pro355
Accession # Q9UBC3
Met1-Pro355
Accession # Q9UBC3
Specificity
Detects human DNMT3B in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human DNMT3B Antibody
Detection of Human DNMT3B by Western Blot.
Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DNMT3B Monoclonal Antibody (Catalog # MAB7646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DNMT3B at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DNMT3B in iPS2 Human Stem Cell Line.
DNMT3B was detected in immersion fixed iPS2 human induced pluripotent stem cell line using Mouse Anti-Human DNMT3B Monoclonal Antibody (Catalog # MAB7646) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DNMT3B in Human Colon.
DNMT3B was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human DNMT3B Monoclonal Antibody (Catalog # MAB7646) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human DNMT3B Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed iPS2 human induced pluripotent stem cell line
Sample: Immersion fixed iPS2 human induced pluripotent stem cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Western Blot
2 µg/mL
Sample: BG01V human embryonic stem cells
Sample: BG01V human embryonic stem cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DNMT3B
DNMT3B (DNA methyltransferase 3B; also DNA MTase HsaIIIB) is a 95-110 kDa member of the C5-methyltransferase family of enzymes. It is ubiquitously expressed in the embryo and found associated with nuclear pericentric heterochromatin. Pericentric heterochromatin is chromatin composed of satellite repeats that lie adjacent to the centromere. DNA (cytosine-guanine dinucleotides) associated with this area undergos methylation, contributing to gene repression and proper chromosome segregation. Human DNMT3B is 853 amino acids (aa) in length. It contains a PWWP (Pro-Trp-Trp-Pro) domain that binds DNA (aa 225-283), a ADD (ATRX, DNMT3, DNMT3L) domain that contains a Zn-finger and binds to an unmethylated histone H3 tail (aa 423-555), and a class I AdoMet-MTase catalytic region that uses S-adenosyl-L-homocysteine as a substrate for methyl transfer (aa 577-847). There are at least five isoform variants. All show a deletion of aa 365-375. In addition, a second contains an alternative start site 12 aa upstream of the standard site, a third shows a deletion of aa 745-807, a fourth contains a Ser substitution for aa 744-853, while a fifth possesses a 45 aa substitution for aa 768-853. Over aa 1-355, human DNMT3B shares 73% aa sequence identity with mouse DNMT3B.
Long Name
DNA [Cytosine-5-]-Methyltransferase 3B
Alternate Names
ICF1, M.HsaIIIB
Gene Symbol
DNMT3B
UniProt
Additional DNMT3B Products
Product Documents for Human DNMT3B Antibody
Product Specific Notices for Human DNMT3B Antibody
For research use only
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