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Human DNMT3B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7646

R&D Systems, part of Bio-Techne
Discontinued Product
MAB7646 has been discontinued. View all DNMT3B products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 832121

Product Specifications

Immunogen

E. coli-derived recombinant human DNMT3B
Met1-Pro355
Accession # Q9UBC3

Specificity

Detects human DNMT3B in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human DNMT3B Antibody

Detection of Human DNMT3B antibody by Western Blot.

Detection of Human DNMT3B by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DNMT3B Monoclonal Antibody (Catalog # MAB7646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DNMT3B at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DNMT3B antibody in iPS2 Human Stem Cell Line by Immunocytochemistry (ICC).

DNMT3B in iPS2 Human Stem Cell Line.

DNMT3B was detected in immersion fixed iPS2 human induced pluripotent stem cell line using Mouse Anti-Human DNMT3B Monoclonal Antibody (Catalog # MAB7646) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DNMT3B antibody in Human Colon by Immunohistochemistry (IHC-P).

DNMT3B in Human Colon.

DNMT3B was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human DNMT3B Monoclonal Antibody (Catalog # MAB7646) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human DNMT3B Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed iPS2 human induced pluripotent stem cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

2 µg/mL
Sample: BG01V human embryonic stem cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNMT3B

DNMT3B (DNA methyltransferase 3B; also DNA MTase HsaIIIB) is a 95-110 kDa member of the C5-methyltransferase family of enzymes. It is ubiquitously expressed in the embryo and found associated with nuclear pericentric heterochromatin. Pericentric heterochromatin is chromatin composed of satellite repeats that lie adjacent to the centromere. DNA (cytosine-guanine dinucleotides) associated with this area undergos methylation, contributing to gene repression and proper chromosome segregation. Human DNMT3B is 853 amino acids (aa) in length. It contains a PWWP (Pro-Trp-Trp-Pro) domain that binds DNA (aa 225-283), a ADD (ATRX, DNMT3, DNMT3L) domain that contains a Zn-finger and binds to an unmethylated histone H3 tail (aa 423-555), and a class I AdoMet-MTase catalytic region that uses S-adenosyl-L-homocysteine as a substrate for methyl transfer (aa 577-847). There are at least five isoform variants. All show a deletion of aa 365-375. In addition, a second contains an alternative start site 12 aa upstream of the standard site, a third shows a deletion of aa 745-807, a fourth contains a Ser substitution for aa 744-853, while a fifth possesses a 45 aa substitution for aa 768-853. Over aa 1-355, human DNMT3B shares 73% aa sequence identity with mouse DNMT3B.

Long Name

DNA [Cytosine-5-]-Methyltransferase 3B

Alternate Names

ICF1, M.HsaIIIB

Entrez Gene IDs

1789 (Human); 13436 (Mouse); 444985 (Rat)

Gene Symbol

DNMT3B

UniProt

Additional DNMT3B Products

Product Documents for Human DNMT3B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DNMT3B Antibody

For research use only

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