Human Dopamine beta-Hydroxylase Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7376

R&D Systems, part of Bio-Techne
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AF7376
AF7376-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Dopamine beta-Hydroxylase Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Dopamine beta‑Hydroxylase
Leu37-Gly617
Accession # P09172

Specificity

Detects human Dopamine beta‑Hydroxylase in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Dopamine beta-Hydroxylase Antibody

Detection of Human Dopamine beta-Hydroxylase antibody by Western Blot.

Detection of Human Dopamine beta-Hydroxylase by Western Blot.

Western blot shows lysates of human adrenal gland tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Dopamine beta-Hydroxylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7376) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dopamine beta-Hydroxylase at approximately 74-77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Dopamine beta-Hydroxylase Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human adrenal gland tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dopamine beta-Hydroxylase

DBH (Dopamine beta-Hydroxylase) is a 77-78 kDa member of the Cu++ type II ascorbate-dependent monooxygenase family of enzymes. It is expressed in noradrenergic nerve terminals and adrenal medullary chromaffin cells, and serves as a catalyst for the conversion of dopamine into norepinepherine. Unlike its family member counterparts (TH and PNMT) that are cytosolic, DBH is embedded in the membranes of secretory vesicles. Human DBH is a 617 amino acid (aa) type II transmembrane glycoprotein (SwissProt # P09172). It contains an N-terminal 16 aa cytoplasmic region, plus a 580 aa lumenal domain (aa 38-617). The luminal region possesses one DOMON (dopamine beta-monooxygenase N-terminal) domain (aa 51-169) plus two consecutive monooxygenase motifs (aa 214-523). DBH exists as both a membrane-embedded 77-78 kDa isoform, and a soluble 73-75 kDa isoform. The latter may arise from cleavage following Gly39. Structurally, DBH will form a disulfide-linked homodimer, which then noncovalently associates with another DBH covalent homodimer, generating a functional 290 kDa homotetramer. Over aa 37‑617, human DBH shares 79% aa sequence identity with mouse DBH.

Alternate Names

DBH, DBM, Dopamine betaHydroxylase, DOPBHY

Entrez Gene IDs

1621 (Human); 13166 (Mouse); 25699 (Rat)

Gene Symbol

DBH

UniProt

P09172

Additional Dopamine beta-Hydroxylase Products

Product Documents for Human Dopamine beta-Hydroxylase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Dopamine beta-Hydroxylase Antibody

For research use only

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