Human EGFR Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF231
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Flow Cytometry, Western Blot
Cited:
ELISA Detection, ELISA Microarray Development, Functional Assay, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EGFR
Leu25-Ser645
Accession # CAA25240
Leu25-Ser645
Accession # CAA25240
Specificity
Detects human EGFR in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse EGFR and less than 0.1% cross-reactivity with recombinant human (rh) EGF, rhErbB2, and rhErbB3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human EGFR Biotinylated Antibody
Detection of EGFR in A431 Human Cell Line by Flow Cytometry.
A431 human epithelial carcinoma cell line was stained with Human EGFR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF231, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).Applications for Human EGFR Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human EGFR (Catalog # 1095-ER)
Sample: Recombinant Human EGFR (Catalog # 1095-ER)
Human EGFR Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using BAF231 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGFR
References
- Daly, R.J. (1999) Growth Factors, 16:255.
- Schlessinger, J. (2000) Cell. 103:211.
- Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
Long Name
Epidermal Growth Factor Receptor
Alternate Names
EGF R, ErbB, ErbB1, HER-1
Gene Symbol
EGFR
UniProt
Additional EGFR Products
Product Documents for Human EGFR Biotinylated Antibody
Product Specific Notices for Human EGFR Biotinylated Antibody
For research use only
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