Human EGFR (Research Grade Matuzumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10023

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems, part of Bio-Techne
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MAB10023-100
MAB10023-SP
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu104

View all EGFR Antibodies »

Product Specifications

Immunogen

Human EGFR

Specificity

Detects human EGFR based on Matuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Matuzumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for Human EGFR (Research Grade Matuzumab Biosimilar) Antibody

Detection of EGF R antibody in A431 human epithelial carcinoma cell line antibody by Flow Cytometry.

Detection of EGF R in A431 human epithelial carcinoma cell line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R (Research Grade Matuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB10023, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human EGFR (Research Grade Matuzumab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/mL
Sample: A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Matuzumab is a biosimilar directed against the extracellular domain of EGFR. Matuzumab binds to epidermal growth factor receptor (EGFR) on the outer membrane of normal and tumor cells. The matuzumab epitope has been mapped to domain III of the extracellular domain of the EGFR.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Human EGFR (Research Grade Matuzumab Biosimilar) Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EGFR (Research Grade Matuzumab Biosimilar) Antibody

For research use only

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